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human m csf  (MedChemExpress)


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    Structured Review

    MedChemExpress human m csf
    Human M Csf, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+m+csf/M-CSF%2C+Human/pmc13050104-38-18-20
    Average 95 stars, based on 4 article reviews
    human m csf - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Purification:

    Article Title: CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma
    Article Snippet: Enrichment efficiency was evaluated by flow cytometry and analyzed with FlowJo. .. Purified TAMs and NTRMs were used immediately for RNA extraction/sequencing or cultured short-term in RPMI-1640 supplemented with 10% FBS (MeilunBio, PWL217), penicillin/streptomycin (MeilunBio, MA0110), and human M-CSF (MCE, HY-P7050). .. Gene silencing was performed using lentiviral shRNAs. shRNAs targeting the human circSMAD4 back-splice junction (and the murine circSmad4 ortholog, avoiding linear Smad4) as well as IGF2BP2 were cloned into the pLKO.1-puro vector (Addgene, #8453).

    Article Title: Colorectal Cancer Cell's Weapon: RNF32 Engages SPP1 + Macrophages to Foster Liver Metastasis, Targeted by Indole‐3‐Acetic Acid
    Article Snippet: CD14‐positive monocytes were then purified from PBMCs using CD14 microbeads (Miltenyi Biotec, Germany) and magnetic‐activated cell sorting (MACS) technology, with a purity exceeding 95% as confirmed by flow cytometry. .. To derive macrophages, the purified monocytes were seeded at a density of 1 × 10 6 cells mL −1 and cultured for 5 days in complete medium supplemented with 50 ng mL −1 human M‐CSF (MedChemExpress, China). ..

    Article Title: CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma
    Article Snippet: Enrichment efficiency was evaluated by flow cytometry and analyzed with FlowJo. .. Purified TAMs and NTRMs were used immediately for RNA extraction/sequencing or cultured short-term in RPMI-1640 supplemented with 10% FBS (MeilunBio, PWL217), penicillin/streptomycin (MeilunBio, MA0110), and human M-CSF (MCE, HY-P7050). .. Gene silencing was performed using lentiviral shRNAs. shRNAs targeting the human circSMAD4 back-splice junction (and the murine circSmad4 ortholog, avoiding linear Smad4) as well as IGF2BP2 were cloned into the pLKO.1-puro vector (Addgene, #8453).

    Article Title: Polydopamine‐Encapsulated Probiotics Restore Gut Homeostasis and Reinstate Macrophage Efferocytosis in Systemic Lupus Erythematosus
    Article Snippet: CD14 + monocytes were then purified from PBMCs using MojoSort Human CD14 Selection Kit according to the manufacturer's instructions (Biolegend, #480025). .. Purified monocytes were cultured in RPMI‐1640 medium supplemented with 10% fetal bovine serum and 50 ng mL −1 recombinant human M‐CSF (MedChemExpress, # HY‐P7050) at 37°C with 5% CO 2 for 7 days to induce differentiation into macrophages. ..

    Article Title: Polydopamine-Encapsulated Probiotics Restore Gut Homeostasis and Reinstate Macrophage Efferocytosis in Systemic Lupus Erythematosus.
    Article Snippet: CD14+ monocytes were then purified from PBMCs using MojoSort Human CD14 Selection Kit according to the manufacturer’s instructions (Biolegend, #480025). .. Purified monocytes were cultured in RPMI1640 medium supplemented with 10% fetal bovine serum and 50 ng mL− 1 recombinant human M-CSF (MedChemExpress, # HY-P7050) at 37◦C with 5% CO2 for 7 days to induce differentiation into macrophages. ..

    Extraction:

    Article Title: CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma
    Article Snippet: Enrichment efficiency was evaluated by flow cytometry and analyzed with FlowJo. .. Purified TAMs and NTRMs were used immediately for RNA extraction/sequencing or cultured short-term in RPMI-1640 supplemented with 10% FBS (MeilunBio, PWL217), penicillin/streptomycin (MeilunBio, MA0110), and human M-CSF (MCE, HY-P7050). .. Gene silencing was performed using lentiviral shRNAs. shRNAs targeting the human circSMAD4 back-splice junction (and the murine circSmad4 ortholog, avoiding linear Smad4) as well as IGF2BP2 were cloned into the pLKO.1-puro vector (Addgene, #8453).

    Article Title: CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma
    Article Snippet: Enrichment efficiency was evaluated by flow cytometry and analyzed with FlowJo. .. Purified TAMs and NTRMs were used immediately for RNA extraction/sequencing or cultured short-term in RPMI-1640 supplemented with 10% FBS (MeilunBio, PWL217), penicillin/streptomycin (MeilunBio, MA0110), and human M-CSF (MCE, HY-P7050). .. Gene silencing was performed using lentiviral shRNAs. shRNAs targeting the human circSMAD4 back-splice junction (and the murine circSmad4 ortholog, avoiding linear Smad4) as well as IGF2BP2 were cloned into the pLKO.1-puro vector (Addgene, #8453).

    Cell Culture:

    Article Title: CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma
    Article Snippet: Enrichment efficiency was evaluated by flow cytometry and analyzed with FlowJo. .. Purified TAMs and NTRMs were used immediately for RNA extraction/sequencing or cultured short-term in RPMI-1640 supplemented with 10% FBS (MeilunBio, PWL217), penicillin/streptomycin (MeilunBio, MA0110), and human M-CSF (MCE, HY-P7050). .. Gene silencing was performed using lentiviral shRNAs. shRNAs targeting the human circSMAD4 back-splice junction (and the murine circSmad4 ortholog, avoiding linear Smad4) as well as IGF2BP2 were cloned into the pLKO.1-puro vector (Addgene, #8453).

    Article Title: Colorectal Cancer Cell's Weapon: RNF32 Engages SPP1 + Macrophages to Foster Liver Metastasis, Targeted by Indole‐3‐Acetic Acid
    Article Snippet: CD14‐positive monocytes were then purified from PBMCs using CD14 microbeads (Miltenyi Biotec, Germany) and magnetic‐activated cell sorting (MACS) technology, with a purity exceeding 95% as confirmed by flow cytometry. .. To derive macrophages, the purified monocytes were seeded at a density of 1 × 10 6 cells mL −1 and cultured for 5 days in complete medium supplemented with 50 ng mL −1 human M‐CSF (MedChemExpress, China). ..

    Article Title: CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma
    Article Snippet: Enrichment efficiency was evaluated by flow cytometry and analyzed with FlowJo. .. Purified TAMs and NTRMs were used immediately for RNA extraction/sequencing or cultured short-term in RPMI-1640 supplemented with 10% FBS (MeilunBio, PWL217), penicillin/streptomycin (MeilunBio, MA0110), and human M-CSF (MCE, HY-P7050). .. Gene silencing was performed using lentiviral shRNAs. shRNAs targeting the human circSMAD4 back-splice junction (and the murine circSmad4 ortholog, avoiding linear Smad4) as well as IGF2BP2 were cloned into the pLKO.1-puro vector (Addgene, #8453).

    Article Title: Polydopamine‐Encapsulated Probiotics Restore Gut Homeostasis and Reinstate Macrophage Efferocytosis in Systemic Lupus Erythematosus
    Article Snippet: CD14 + monocytes were then purified from PBMCs using MojoSort Human CD14 Selection Kit according to the manufacturer's instructions (Biolegend, #480025). .. Purified monocytes were cultured in RPMI‐1640 medium supplemented with 10% fetal bovine serum and 50 ng mL −1 recombinant human M‐CSF (MedChemExpress, # HY‐P7050) at 37°C with 5% CO 2 for 7 days to induce differentiation into macrophages. ..

    Article Title: IL-33 accelerates orthodontic tooth movement by promoting M1 differentiation of macrophages and osteoclast formation.
    Article Snippet: CD14+ monocytes were isolated from PBMCs using anti-CD14-conjugated microbeads. .. Cells were cultured in α-MEM supplemented with 10% FBS, 2 mM L-glutamine, 100 U/ml penicillinstreptomycin, and 10 ng/ml human M-CSF (HY-P7050, MCE) to induce osteoclast differentiation. .. Human RANKL (HY-P73386, MCE) (20 ng/ml), human IL-33 (50 ng/ml) (HY-P70475, MCE), and anti-human ST2 antibody (HY-P87275, MCE) (5 μg/ml) were added to the culture medium.

    Article Title: Polydopamine-Encapsulated Probiotics Restore Gut Homeostasis and Reinstate Macrophage Efferocytosis in Systemic Lupus Erythematosus.
    Article Snippet: CD14+ monocytes were then purified from PBMCs using MojoSort Human CD14 Selection Kit according to the manufacturer’s instructions (Biolegend, #480025). .. Purified monocytes were cultured in RPMI1640 medium supplemented with 10% fetal bovine serum and 50 ng mL− 1 recombinant human M-CSF (MedChemExpress, # HY-P7050) at 37◦C with 5% CO2 for 7 days to induce differentiation into macrophages. ..

    Recombinant:

    Article Title: Polydopamine‐Encapsulated Probiotics Restore Gut Homeostasis and Reinstate Macrophage Efferocytosis in Systemic Lupus Erythematosus
    Article Snippet: CD14 + monocytes were then purified from PBMCs using MojoSort Human CD14 Selection Kit according to the manufacturer's instructions (Biolegend, #480025). .. Purified monocytes were cultured in RPMI‐1640 medium supplemented with 10% fetal bovine serum and 50 ng mL −1 recombinant human M‐CSF (MedChemExpress, # HY‐P7050) at 37°C with 5% CO 2 for 7 days to induce differentiation into macrophages. ..

    Article Title: Polydopamine-Encapsulated Probiotics Restore Gut Homeostasis and Reinstate Macrophage Efferocytosis in Systemic Lupus Erythematosus.
    Article Snippet: CD14+ monocytes were then purified from PBMCs using MojoSort Human CD14 Selection Kit according to the manufacturer’s instructions (Biolegend, #480025). .. Purified monocytes were cultured in RPMI1640 medium supplemented with 10% fetal bovine serum and 50 ng mL− 1 recombinant human M-CSF (MedChemExpress, # HY-P7050) at 37◦C with 5% CO2 for 7 days to induce differentiation into macrophages. ..

    Isolation:

    Article Title: CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma
    Article Snippet: .. CD14 + monocytes were isolated and differentiated in RPMI-1640 medium (MeilunBio, MA0215) containing 10% FBS (MeilunBio, PWL217) and human M-CSF (MCE, HY-P7050) for 7 days. .. Mouse bone marrow–derived macrophages (BMDMs) were generated from C57BL/6 mice (6–8 weeks old) by culturing bone marrow cells in high-glucose DMEM (MeilunBio, MA0212) supplemented with 10% FBS (MeilunBio, PWL217) and mouse M-CSF (MCE, HY-P7085) for 7 days.

    Article Title: CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinoma
    Article Snippet: .. CD14+ monocytes were isolated and differentiated in RPMI-1640 medium (MeilunBio, MA0215) containing 10% FBS (MeilunBio, PWL217) and human M-CSF (MCE, HYP7050) for 7 days. .. Mouse bone marrow–derived macrophages (BMDMs) were generated from C57BL/6 mice (6–8 weeks old) by culturing bone marrow cells in high-glucose DMEM (MeilunBio, MA0212) supplemented with 10% FBS (MeilunBio, PWL217) and mouse M-CSF (MCE, HY-P7085) for 7 days.



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